Name細(xì)胞名稱:NTCC? AG08033 EQUUS | ORDER: PERISSODACTYLA Aorta, thoracic Cell Line雅科仕 |目: 奇蹄目細(xì)胞株Description描述:Standardbred horse. This line is a second cloned culture from the same population of cells as AG8033. The thoracic aortawas removed post-mortem on 2/26/85. The primary culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The culture was cloned at PDL 5, expanded and frozen. The cell morphology is polygonal. Culture submitted by F Angelosanto (#0477). The culture was grown on gelatincoated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: AMMC 20U (with supplements). This cell line will only be shipped frozen.Category分類:EndothelialID編號:NTCC?-AG08033Size/Quantity數(shù)量: 1 Flask/2 VialsBiosafety Level生物安全級別:1Shipping Info運(yùn)輸方式: RT/Dry IceStorage儲(chǔ)存方式: RT/Liquid NitrogenRace: Age年齡:6 YRGender性別:FemaleCell Type細(xì)胞類型: EQUUS | ORDER: PERISSODACTYLAAffected State:NoProduct:EndothelialGene:Mutations突變:Ethnicity:Family:1094Relationship:probandKaryotype:Tissue Type組織來源:ArteryComplete Growth Medium完全培養(yǎng)基: BioVector? MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent Complete MediumSubculturing傳代方法:Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phaseCulture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°CSTR Profile鑒定數(shù)據(jù):References參考文獻(xiàn):Supplier供應(yīng)商:BioVector質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
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