Name細胞名稱：NTCC? AG11132 APPARENTLY HEALTHY INDIVIDUAL Breast organoid Cell Line
明顯健康的個人細胞株
Description描述：Clinically unremarkable breast tissue was obtained in 1978 during reduction mammoplasty. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 7/88 from thawed organoids by explantation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 48R/AC170 at passage 8, PDL 30 (estimated by submitter), and has been frozen at the Repository at PDL 45. Culture is grown in serum-free medium.
Category分類：Epithelial
ID編號:NTCC?-AG11132
Size/Quantity數量: 1 Flask/2 Vials
Biosafety Level生物安全級別:1
Shipping Info運輸方式: RT/Dry Ice
Storage儲存方式: RT/Liquid Nitrogen
Race: Black/African American
Age年齡:16 YR
Gender性別:Female
Cell Type細胞類型: APPARENTLY HEALTHY INDIVIDUAL
Affected State:No
Product:Epithelial
Gene:
Mutations突變:
Ethnicity:
Family:
Relationship:proband
Karyotype:46,XX
Tissue Type組織來源:Breast
Complete Growth Medium完全培養基: BioVector? MEGM-mammary epithelial cell medium plus supplements Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數據:
References參考文獻:

Supplier供應商:BioVector質粒載體菌株細胞蛋白抗體基因保藏中心
NTCC典型培養物保藏中心
Tel電話：400-800-2947
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