Name細(xì)胞名稱(chēng)：NTCC? AG11136 APPARENTLY HEALTHY INDIVIDUAL Breast organoid Cell Line
明顯健康的個(gè)人細(xì)胞株
Description描述：Tissue was obtained in 1981 during subcutaneous mastectomy of breast contralateral to breast removed for tumor; pathologic diagnosis was mild fibrocystic disease. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 2/85 from thawed organoids by explantation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 210C/AB470 at passage 5, PDL 22 (estimated by submitter), and has been frozen at the Repository at PDL 30. Culture is grown in serumfree medium. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN
Category分類(lèi)：Epithelial
ID編號(hào):NTCC?-AG11136
Size/Quantity數(shù)量: 1 Flask/2 Vials
Biosafety Level生物安全級(jí)別:1
Shipping Info運(yùn)輸方式: RT/Dry Ice
Storage儲(chǔ)存方式: RT/Liquid Nitrogen
Race: White
Age年齡:42 YR
Gender性別:Female
Cell Type細(xì)胞類(lèi)型: APPARENTLY HEALTHY INDIVIDUAL
Affected State:No
Product:Epithelial
Gene:
Mutations突變:
Ethnicity:
Family:
Relationship:proband
Karyotype:
Tissue Type組織來(lái)源:Breast
Complete Growth Medium完全培養(yǎng)基: BioVector? MEGM-mammary epithelial cell medium plus supplements Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻(xiàn):

Supplier供應(yīng)商:BioVector質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
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