Name細胞名稱：NTCC? AG12120 SUS | ORDER: CETARTIODACTYLA Aorta, thoracic Cell Line
不銹鋼|目：鯨齒獸細胞株
Description描述：Yorkshire breed. The thoracic aorta was removed immediately post-mortem on 3/12/92. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: HF12 with 10% plasma-derived donor horse serum with supplements. The HF12 medium used should be endotoxin-free. Other cultures from this pig are AG12077 and AG12119. The karyotype is 38,XX with 2% of the cells examined showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分類：Endothelial
ID編號:NTCC?-AG12120
Size/Quantity數量: 1 Flask/2 Vials
Biosafety Level生物安全級別:1
Shipping Info運輸方式: RT/Dry Ice
Storage儲存方式: RT/Liquid Nitrogen
Race:
Age年齡:10 WK
Gender性別:Female
Cell Type細胞類型: SUS | ORDER: CETARTIODACTYLA
Affected State:No
Product:Endothelial
Gene:
Mutations突變:
Ethnicity:YORKSHIRE
Family:1446
Relationship:proband
Karyotype:
Tissue Type組織來源:Artery
Complete Growth Medium完全培養基: BioVector? Ham's F12 with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數據:
References參考文獻:

Supplier供應商:BioVector質粒載體菌株細胞蛋白抗體基因保藏中心
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