Name細胞名稱：NTCC? AG08594 BOS | ORDER: CETARTIODACTYLA Aorta, thoracic Cell Line
博斯|目：鯨齒獸細胞株
Description描述：Angus breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 12/3/85. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The karyotype is 60,XY; normal diploid male. The first and second passage cells were propagated in medium supplemented with horse plasma-derived serum and heparin (0.1mg/ml) on gelatin-coated flasks. The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). Cells were frozen in medium supplemented with fetal bovine serum. Culture medium: AMMC 20U with supplements. For lifespan determination this cell line was cultured in Ham's F12 medium with 10% inactivated fetal bovine serum. A smooth muscle cell culture from this calf is AG08595A. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分類：Endothelial
ID編號:NTCC?-AG08594
Size/Quantity數量: 1 Flask/2 Vials
Biosafety Level生物安全級別:1
Shipping Info運輸方式: RT/Dry Ice
Storage儲存方式: RT/Liquid Nitrogen
Race:
Age年齡:1 YR
Gender性別:Male
Cell Type細胞類型: BOS | ORDER: CETARTIODACTYLA
Affected State:No
Product:Endothelial
Gene:
Mutations突變:
Ethnicity:
Family:2068
Relationship:proband
Karyotype:60,XY
Tissue Type組織來源:Artery
Complete Growth Medium完全培養基: BioVector? Ham's F12 medium/Medium 199, 1:1 mixture with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數據:
References參考文獻:

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