Name細(xì)胞名稱：NTCC? GM21082 BETA-THALASSEMIA | HEMOGLOBIN--BETA LOCUS; HBB B-Lymphocyte Cell Line
β-地中海貧血 |血紅蛋白--β基因座；六溴代二苯細(xì)胞株
Description描述：Clinically affected; Hemoglobin E/beta thalassemia; diagnosed at birth; transfusion dependent since age 3 years; iron overload; splenectomy at age 3 years; history of slow growth development with use of growth hormones for about 1 year; history of delayed sexual development with testosterone replacement for about 1 year; melanoma diagnosed at age 28 years; high frequency hearing loss; history of respiratory infections and pneumonia in childhood; hemoglobin electrophoresis results: HbA = 91.2%, HbA2 = 0%, HbF = 1.3%, HbS = 0%, HbE = 7.5%; hemoglobin = 12.4 g/dl; MCV = 84.4; donor subject has a G>A change in codon 26 of the HBB gene resulting in the substitution of lysine for glutamic acid [Glu26Lys (E26K)]; transfusion-dependent; currently on iron chelation therapy_x000D_
Category分類：B-Lymphocyte
ID編號:NTCC?-GM21082
Size/Quantity數(shù)量: 1 Flask/2 Vials
Biosafety Level生物安全級別:1
Shipping Info運(yùn)輸方式: RT/Dry Ice
Storage儲(chǔ)存方式: RT/Liquid Nitrogen
Race: Asian, Other
Age年齡:29 YR
Gender性別:Male
Cell Type細(xì)胞類型: BETA-THALASSEMIA | HEMOGLOBIN--BETA LOCUS; HBB
Affected State:Yes
Product:LCL
Gene:HBB
Mutations突變:GLU26LYS (GLU27LYS; c.79G>A)
Ethnicity:VIETNAMESE/PANAMANIAN/CHINESE
Family:
Relationship:proband
Karyotype:
Tissue Type組織來源:Blood
Complete Growth Medium完全培養(yǎng)基: BioVector? Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻(xiàn):

Supplier供應(yīng)商:BioVector質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
NTCC典型培養(yǎng)物保藏中心
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