• History
  Kc line established from 6-12 hr embryos of cross se x e. Clone 167 isolated in Goldschmitt-Clermont laboratory. Selected for growth in high-pH medium by Bourouis and Jarry. Restored to normal medium in Cherbas laboratory
  2007/02/19 15:39:39
• Sex
  Female, by criterion of dsx splicing (Lynch and Maniatis, 1996). The assignment was confirmed by criteria of the expression levels of roX gene, msl-2 and traF, and the splicing patterns of tra and Sxl (Lee et al., 2014), and again by roX gene expression and Sxl splicing (Stoiber et al., 2016, G3)
  2019/05/15 14:49:18
• Karyotype
  XO-haplo-IV pseudodiploid (Cherbas lab, unpublished)
  2007/02/19 15:40:56
• recommended media
  Kc167 can be grown in M3 plus BPYE plus 5% fetal calf serum. If you substitute Schneider's medium for M3 plus BPYE, the cells will grow, but more slowly. If you increase the serum concentration, it will do no harm, but it is a waste of money. In our hands, the line does best in CCM-3, a serum-free medium from HyClone. HyClone is phasing out CCM-3, and replacing it in their catalog with an "updated" version called SFX, also used without the addition of serum. In our tests, Kc167 cells grow distinctly better in CCM-3 than in SFX. As of now (January, 2014), CCM-3 is still available from HyClone, but there is a 10-week wait for orders of the powdered medium.
  2014/01/19 10:55:53
• tissue source
  Transcriptome analysis suggests plasmatocyte-like properties for Kc167 cells (Cherbas et al., 2011)
  2011/03/09 16:13:47
• modENCODE data
  Kc167 is one of the four lines chosen for extensive analysis by the modENCODE project. Data on transcripts, protein binding sites and replication patterns are posted on FlyBase; transcriptome data are also available through the expression tool on the DGRC site.

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