Synonyms: SL2 , Schneider's 2 , Schneider's line 2

Features:

• authenticated

Categories:

• Dm Embryonic

This is the line which we formerly referred to as simply S2. We have changed its name in an effort to reduce the confusion in the literature about multiple isolates of the original Schneider's line 2.

• Cell Maintenance
  Maintain S2 cell line between 2.0 x 10e6 cells/mL - 1.0 x 10e7 cells/mL.
  2010/08/12 15:17:05
• History
  Made from Oregon R embryos on the verge of hatching. This isolate was a gift from Cheeptip Benyajati to the Cherbas lab, ca. 1980.
  2016/05/16 00:44:18
• Sex
  Male, by criterion of MSL complex assembly
  2007/02/19 05:38:49
• Note
  MSL complex assembles on the X chromosome, implying a male character (Copps et al., 1998).
  2007/02/19 05:38:49
• S2 stocks free from mad cow disease
  Orders for S2 are normally filled from stocks of cells grown and frozen in medium containing recent lots of fetal calf serum of United States origin. We have a small stock of S2 cells from ampoules frozen prior to 2004 (the first known appearance of mad cow disease in the U.S.), then thawed and expanded in medium containing Australian serum (free from mad cow disease), and refrozen in a serum-free freezing medium. This stock is intended for users who intend to use the cells for pharmaceutical purposes, and who are legally required to use cells that have no possibility of contamination from mad cow disease.