Cell Line Name：NTCC? HPAF-II-SpCas9 Stable Cell Line
Human cell line HPAF-II stably expressing Cas9?from random site
人細(xì)胞株 HPAF-II 隨機(jī)位點穩(wěn)定表達(dá) Cas9胰腺癌
Selection Marker篩選標(biāo)記：Hygro
Cat#貨號:NTCC?-SL528
Size/Quantity數(shù)量: 1 Flask / 2 Vials
Biosafety Level生物安全級別: 1
Shipping Info運(yùn)輸方式: RT/Dry Ice
Storage儲存方式: RT/Liquid Nitrogen
Description描述:BioVector NTCC Inc.’s stable cell lines constitutively expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications with high efficiency. The NTCC? Cas9 stable cell lines are available pre-made in many human, mouse, and rat cell line backgrounds. The S. pyogenes CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 “Safe Harbor” sites, or randomly via lentiviral-mediated transduction. The CRISPR Cas9 nuclease, in combination with single guide RNAs (sgRNAs), is widely used to create targeted genomic modifications, from gene knockouts, mutagenesis, fusion tagging, and more, in eukaryotic cells and animal models. Stable Cas9 integration ensures robust and consistent expression of the CRISPR Cas9 nuclease. Advantages Stable Cas9 integration minimizes the need for co-transfection or co-transduction of sgRNAs, ideal for high-throughput sgRNA applications. Single clone isolation provides consistent, high-level Cas9 expression in a uniform genetic background. Cas9 functionally validated using the NTCC? T7 Endonuclease I assay. Compatible with BioVector NTCC Inc. NTCC? sgRNA clones, sgRNA libraries, and donor clones.
Gender性別:
Other Collection No.其他編號:
Cell Type細(xì)胞類型: Pancreatic cancer
Source組織來源:
Specification規(guī)格:
Cell Images細(xì)胞圖片:
Complete Growth Medium完全培養(yǎng)基: NTCC? HPAF-II-SpCas9 Stable Cell Line Complete Medium
Subculturing傳代方法:
Cryopreservation凍存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: RT/Liquid nitrogen vapor phase
Culture Conditions培養(yǎng)條件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鑒定數(shù)據(jù):
References參考文獻(xiàn):

Supplier供應(yīng)商:
BioVector質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
NTCC典型培養(yǎng)物保藏中心
Tel電話：400-800-2947
Email：Biovector@163.com
http://www.biovector.net