32D/MPL NTCC?小鼠骨髓淋巴母細胞穩轉株32DMPL Cell line-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

Resource Identification Initiative To cite this cell line use: Mu-mpl 32D (NTCC?_C7PN)

CommentsCharacteristics: IL3 dependent.

Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:97076; Mpl.

Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.

Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.

Species of originMus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: C3H/HeJ.

HierarchyParent: NTCC?_6G54 (32Dcl23)

Sex of cell Male

Category Factor-dependent cell line

Cell pictures:

Preclinical in vivo assessment of the pharmacologic activity of nonpeptidyl thrombopoietin receptor (TPOR) agonists is very difficult because of the high species specificity of such agonists. In this study, we have developed a novel and simple in vivo hollow-fiber assay to preclinically evaluate TPOR agonists. The 32D-mpl cell line was generated by stable transfection of human TPOR into 32D lymphoblast cells and shown to be a specific model for nonpeptide TPOR agonists in vitro. Stably transfected 32D-mpl cells were then sealed in hollow fibers and implanted into nude mice. Cells in hollow fibers specifically responded to TPOR agonists, including thrombopoietin and eltrombopag, a nonpeptide small-molecule TPOR agonist, but not to granulocyte colony-stimulating factor or erythropoietin. Oral administration of eltrombopag stimulated 32D-mpl cell proliferation, prevented 32D-mpl cell apoptosis, and stimulated the phosphorylation of cellular signaling transducers and activators of transcription in a TPOR- and dose-dependent manner. These results indicate that the hollow-fiber assay is a specific and efficient model for rapidly evaluating the in vivo activity of small-molecule TPOR agonists.

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