BCP-1 NTCC?人體腔淋巴瘤細胞株-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心
- 價 格:¥49950
- 貨 號:NTCC?-2294 BCP-1
- 產 地:北京
- BioVector NTCC典型培養物保藏中心
- 聯系人:Dr.Xu, Biovector NTCC Inc.
電話:400-800-2947 工作QQ:1843439339 (微信同號)
郵件:Biovector@163.com
手機:18901268599
地址:北京
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BCP-1 NTCC?人體腔淋巴瘤細胞株-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心
BCP-1 is a B lymphoblast cell that was isolated from the peripheral blood of a patient with body cavity-based lymphoma. This cell has applications in immunology research.
- Product category
- Human cells
- Organism
- Homo sapiens, human
- Cell type
- B lymphoblast
- Morphology
- lymphoblast
- Tissue
- Peripheral blood
- Disease
- Body Cavity Based Lymphoma
- Applications
- 3D cell cultureImmunology
- Product format
- Frozen
Characteristics
- Growth properties
- Suspension
- Derivation
- BCP-1 is a clonal lymphoma cell line isolated in 1995 by serial dilution of peripheral blood mononuclear cells from an HIV seronegative patient with body cavity based lymphoma (BCBL).
- Age
- 92 years
- Gender
- Male
- Comments
The BCP-1 cell line is positive for the Kaposi's sarcoma associated herpesvirus (KSHV, provisionally designated Human Herpes Virus-8 or HHV-8) genome at 150 copies/cell.
BCP-1 is negative for Epstein-Barr virus (EBV), HHV-6 and HHV-7.
Handling information
- Unpacking and storage instructions
Check all containers for leakage or breakage.
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below --130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
- Temperature
- 37°C
- Atmosphere
- 95% Air, 5% CO2
- Handling procedure
- To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.
Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
- Subculturing procedure
- Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.Interval: Maintain between 1 x 105 and 1 x 106 cells/mL.Medium Renewal: Every 2 to 3 days
- Reagents for cryopreservation
- Complete growth medium supplemented with 7.5% (v/v) DMSO (ATCC 4-X)
Quality control specifications
- Mycoplasma contamination
- Not detected
- STR profiling
- Amelogenin: X
CSF1PO: 10
D13S317: 11,13
D16S539: 10,11
D5S818: 12
D7S820: 10,11
TH01: 7,9
TPOX: 8,9
vWA: 17,18
D3S1358: 16,17
D21S11: 28,31.2
D18S51: 13,17
Penta_E: 10
Penta_D: 8,13
D8S1179: 14
FGA: 20,22.2
D19S433: 14,15
D2S1338: 23,24
History
- Deposited as
- Homo sapiens
- Depositors
- SJ Gao, PS Moore, Y Chang
- Year of origin
- 1995
- Special collection
- NCRR Contract
Supplier: BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心
www.biovector.net
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