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首頁 ? BCP-1 NTCC?人體腔淋巴瘤細胞株-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

BCP-1 NTCC?人體腔淋巴瘤細胞株-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

  • 價  格:¥49950
  • 貨  號:NTCC?-2294 BCP-1
  • 產  地:北京
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BCP-1 NTCC?人體腔淋巴瘤細胞株-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心


BCP-1 is a B lymphoblast cell that was isolated from the peripheral blood of a patient with body cavity-based lymphoma. This cell has applications in immunology research.

BCP-1 cells circulating through and forming microscopic tumor deposits... |  Download Scientific Diagram

Product category
Human cells
Organism
Homo sapiens, human
Cell type
B lymphoblast
Morphology
lymphoblast
Tissue
Peripheral blood
Disease
Body Cavity Based Lymphoma
Applications
3D cell culture
Immunology
Product format
Frozen

Characteristics

Growth properties
Suspension
Derivation
BCP-1 is a clonal lymphoma cell line isolated in 1995 by serial dilution of peripheral blood mononuclear cells from an HIV seronegative patient with body cavity based lymphoma (BCBL).
Age
92 years
Gender
Male
Comments

The BCP-1 cell line is positive for the Kaposi's sarcoma associated herpesvirus (KSHV, provisionally designated Human Herpes Virus-8 or HHV-8) genome at 150 copies/cell.

BCP-1 is negative for Epstein-Barr virus (EBV), HHV-6 and HHV-7.

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.

  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below --130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.
  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

  3. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.

  4. Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 culture flask.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

  5. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.

Subculturing procedure
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Interval: Maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Reagents for cryopreservation
Complete growth medium supplemented with 7.5% (v/v) DMSO (ATCC 4-X)

Quality control specifications

Mycoplasma contamination
Not detected
STR profiling
Amelogenin: X
CSF1PO: 10
D13S317: 11,13
D16S539: 10,11
D5S818: 12
D7S820: 10,11
TH01: 7,9
TPOX: 8,9
vWA: 17,18
D3S1358: 16,17
D21S11: 28,31.2
D18S51: 13,17
Penta_E: 10
Penta_D: 8,13
D8S1179: 14
FGA: 20,22.2
D19S433: 14,15
D2S1338: 23,24

History

Deposited as
Homo sapiens
Depositors
SJ Gao, PS Moore, Y Chang
Year of origin
1995
Special collection
NCRR Contract

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