IncP RK2 BioVector?大腸桿菌、銅綠假單胞菌和克雷伯氏肺炎菌等廣宿主穿梭質(zhì)粒載體

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

IncP RK2 Plasmid: A Versatile Genetic Tool

The IncP RK2 plasmid is a remarkable piece of DNA that has captivated the attention of microbiologists and genetic engineers alike. Its unique properties and wide host range make it a valuable tool for various applications in biotechnology and research.

Key Characteristics:

• Broad Host Range: RK2 can replicate and transfer itself between a wide range of Gram-negative bacteria, including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. This versatility makes it a powerful tool for genetic engineering experiments.

• Self-Transmissible: RK2 carries genes that enable it to transfer itself from one bacterium to another through conjugation, a process involving direct cell-to-cell contact. This property allows for rapid dissemination of genetic information.

• Antibiotic Resistance: RK2 often carries genes conferring resistance to multiple antibiotics, such as ampicillin, kanamycin, and tetracycline. These resistance genes can be used as selectable markers in genetic engineering experiments.

• Complex Regulatory Network: RK2 possesses a sophisticated regulatory system that controls its replication, transfer, and maintenance. This system ensures that the plasmid is stably inherited by daughter cells and efficiently transferred to new hosts.

Applications:

• Genetic Engineering: RK2-based vectors are widely used to introduce foreign genes into bacteria. Its broad host range allows for genetic manipulation of a variety of bacterial species.

• Molecular Biology Research: RK2 plasmids are valuable tools for studying plasmid replication, transfer, and gene expression.

• Biotechnology: RK2 can be used to express recombinant proteins in bacteria, making it a useful tool for industrial biotechnology.

The IncP RK2 plasmid continues to be a valuable asset in the field of microbiology and biotechnology. Its unique properties and versatility have made it an indispensable tool for researchers and scientists worldwide.

IncP RK2 質(zhì)粒：一種多功能遺傳工具

IncP RK2 質(zhì)粒是一種引人注目的 DNA 片段，吸引了微生物學(xué)家和遺傳工程師的廣泛關(guān)注。其獨(dú)特的特性和廣泛的宿主范圍使其成為生物技術(shù)和研究領(lǐng)域各種應(yīng)用的有價(jià)值工具。

[IncP RK2 質(zhì)粒的圖譜]

主要特征：

• 廣泛的宿主范圍： RK2 可以在多種革蘭氏陰性細(xì)菌之間復(fù)制和轉(zhuǎn)移自身，包括 大腸桿菌、銅綠假單胞菌 和 克雷伯氏肺炎菌。這種多功能性使其成為遺傳工程實(shí)驗(yàn)的強(qiáng)大工具。

• 自傳遞性： RK2 攜帶基因，使其能夠通過接合（一種涉及直接細(xì)胞間接觸的過程）將自身從一個細(xì)菌轉(zhuǎn)移到另一個細(xì)菌。這種特性允許快速傳播遺傳信息。

• 抗生素抗性： RK2 通常攜帶賦予多種抗生素抗性的基因，如氨芐青霉素、卡那霉素和四環(huán)素。這些抗性基因可用作遺傳工程實(shí)驗(yàn)中的選擇標(biāo)記。

• 復(fù)雜的調(diào)控網(wǎng)絡(luò)： RK2 擁有一個復(fù)雜的調(diào)控系統(tǒng)，控制其復(fù)制、轉(zhuǎn)移和維持。該系統(tǒng)確保質(zhì)粒被子細(xì)胞穩(wěn)定遺傳并有效轉(zhuǎn)移到新宿主。

應(yīng)用：

• 遺傳工程： 基于 RK2 的載體廣泛用于將外源基因引入細(xì)菌。其廣泛的宿主范圍允許對多種細(xì)菌物種進(jìn)行基因操作。

• 分子生物學(xué)研究： RK2 質(zhì)粒是研究質(zhì)粒復(fù)制、轉(zhuǎn)移和基因表達(dá)的有價(jià)值工具。

• 生物技術(shù)： RK2 可用于在細(xì)菌中表達(dá)重組蛋白，使其成為工業(yè)生物技術(shù)的有用工具。

IncP RK2 質(zhì)粒繼續(xù)成為微生物學(xué)和生物技術(shù)領(lǐng)域的重要資產(chǎn)。其獨(dú)特的特性和多功能性使其成為全球研究人員和科學(xué)家不可或缺的工具。

生產(chǎn)廠家Supplier:

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

E-mail:BioVector@163.com

http://www.biovector.net