JC53bl NTCC?人乳頭瘤病毒相關的宮頸腺癌細胞株-用于HIV感染報告細胞株-BioVector NTCC細胞保藏中心

JC53bl cells, also known as TZM-bl cells, are a widely used cell line in HIV research. They are derived from HeLa cells and engineered to express CD4, CCR5, and CXCR4, which are the receptors that HIV-1 uses to enter cells. JC53bl cells also contain reporter genes that allow for the detection of HIV infection.

JC53bl cells

Key features and applications:

• HIV research: JC53bl cells are used to study HIV infection, replication, and neutralization. They are particularly useful for testing the effectiveness of anti-HIV drugs and vaccines.

• Neutralizing antibody assays: These cells are used to measure the ability of antibodies to neutralize HIV-1. This information is important for evaluating the effectiveness of HIV vaccines and for monitoring the development of HIV-specific antibodies in infected individuals.

• Drug discovery: JC53bl cells are used to screen for new anti-HIV drugs. They can also be used to study the mechanisms of action of existing drugs.

ARP-8129 is a HeLa cell line generated from JC.53 cells by introducing separate integrated copies of the luciferase and β-galactosidase genes under control of the human immunodeficiency virus type 1 (HIV-1) promoter.

The TZM-bl cell line, previously designated JC53-bl (clone 13), is an indicator cell line that is highly sensitive to infection with diverse isolates of HIV-1 and enables quantitative analysis of HIV infection using either β-galactosidase or luciferase as a reporter. The parental cell line (JC.53) stably expresses large amounts of CD4 and CCR5 and constitutively expresses CXCR4.

ARP-8129 is contaminated with ecotropic murine leukemia virus (MLV). For additional information, please consult the references below.

ARP-8129 is an adherent cell line with no special requirements for thawing and reestablishing the culture. ARP-8129 grows in a single cell layer. Antibiotic selection is not required to maintain stable expression of the receptors and reporter genes.

The recommended propagation medium is DMEM supplemented with 10% FBS, 100 U/mL penicillin and 0.1 mg/mL streptomycin.

Tests for bacteria, fungi and mycoplasma were negative.

Each vial of ARP-8129 contains approximately 5.95 × 106 cells in 1 mL of fetal bovine serum (FBS) supplemented with 40% DMEM and 10% dimethyl sulfoxide (DMSO). Post-thaw viability was 85.9%.

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