pMGBm19 BioVector?巨大芽孢桿菌木糖誘導型高效表達載體-BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

BioVector?pMGBm19 is a plasmid vector designed for use in Bacillus megaterium, a bacterium known for its ability to produce high levels of recombinant proteins. It's part of a series of vectors specifically developed for this organism.

Key Features of pMGBm19:

• Shuttle Vector: It can replicate in both Escherichia coli and Bacillus megaterium, allowing for convenient cloning and expression in both organisms.

• Xylose-Inducible Promoter: The PxylA promoter enables controlled expression of the gene of interest by adding xylose to the growth medium.

• Multiple Cloning Sites (MCS): The MCS allows for flexible insertion of the gene of interest.

• Selection Markers: The vector carries antibiotic resistance genes for selection in both E. coli and B. megaterium.

Applications:

• Protein Production: High-level production of recombinant proteins in B. megaterium.

• Metabolic Engineering: Engineering B. megaterium strains for the production of valuable compounds.

• Synthetic Biology: Constructing synthetic biological circuits in B. megaterium.

Advantages of Using pMGBm19:

• High Protein Yield: B. megaterium is known for its ability to produce high levels of recombinant proteins.

• Secretion Capability: The vector can be used to express proteins that are secreted into the culture medium, simplifying purification.

• Robustness: B. megaterium is a robust organism that can grow in a variety of conditions.

Plasmid map:

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BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

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