pRS406 BioVector?酵母整合型表達載體

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

pRS406 is a yeast integrative vector, commonly used in genetic engineering experiments involving the budding yeast Saccharomyces cerevisiae.

Key Features:

• Integrative Vector: Designed to integrate into the yeast genome through homologous recombination.

• URA3 Marker: Contains the URA3 gene, which allows for selection of yeast cells that have successfully integrated the plasmid. URA3 encodes an enzyme involved in uracil biosynthesis, enabling yeast cells carrying the plasmid to grow on media lacking uracil.

• Multiple Cloning Site (MCS): Includes a region with various restriction enzyme recognition sites, facilitating the insertion of foreign DNA fragments.

• Ampicillin Resistance Gene: Contains the AmpR gene, conferring resistance to ampicillin in Escherichia coli (E. coli). This allows for selection and propagation of the plasmid in E. coli before transformation into yeast.

Applications:

• Gene Disruption: Used to disrupt or delete specific genes in yeast by integrating the plasmid into the target gene locus.

• Gene Tagging: Can be used to add tags (such as fluorescent proteins or epitope tags) to proteins of interest.

• Gene Overexpression: Can be used to overexpress genes in yeast by integrating the plasmid into a high-copy number locus.

• Gene Replacement: Used to replace a specific gene with a modified version.

Significance:

pRS406 and other vectors in the pRS series have been instrumental in advancing yeast genetics research. They provide a versatile platform for manipulating the yeast genome, enabling scientists to study gene function, develop new strains, and investigate various biological processes.

pRS406 plasmid map

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