pNL1.1 CMV Nluc BioVector?啟動子Nluc熒光報告載體

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

pNL1.1 CMV Nluc is a reporter vector used in molecular biology to study gene expression and promoter activity.

Key Components:

• CMV Promoter: The Cytomegalovirus (CMV) immediate-early promoter/enhancer drives the expression of the reporter gene.

• NanoLuc Luciferase Gene (Nluc): This gene encodes NanoLuc, a small and bright luciferase enzyme that produces luminescence upon addition of its substrate, furimazine.

• Ampicillin Resistance Gene (AmpR): Confers resistance to the antibiotic ampicillin, allowing for selection of bacteria containing the plasmid.

• Origin of Replication (ori): Enables replication of the plasmid in bacteria.

Applications:

• Promoter Analysis: Used to study the activity of promoters and enhancers by cloning them upstream of the Nluc gene. The intensity of luminescence correlates with the strength of the promoter.

• Gene Expression Studies: Can be used to monitor gene expression levels in response to various stimuli, such as drugs, hormones, or growth factors.

• Drug Screening: Employed in drug discovery to identify compounds that modulate gene expression.

• Viral Vector Development: Used to assess the efficiency of viral vectors in delivering genes to target cells.

Advantages:

• High Sensitivity: NanoLuc luciferase is highly sensitive, allowing for the detection of even low levels of gene expression.

• Fast and Easy Assays: Luminescence assays are rapid and simple to perform, providing quick results.

• Versatility: The CMV promoter can be replaced with other promoters of interest, making the vector adaptable to various research applications.

Significance:

pNL1.1 CMV Nluc and other reporter vectors have become indispensable tools in molecular biology research. They provide a powerful and versatile platform for studying gene expression and function, contributing to advancements in various fields, including drug discovery, gene therapy, and basic research.

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