pINA1292 BioVector?解脂耶氏酵母整合型高表達載體

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

pINA1292 is a plasmid commonly used in the yeast Yarrowia lipolytica for protein expression and secretion. It's a multi-copy integrative vector, meaning it can integrate multiple copies into the yeast genome, potentially leading to higher protein production levels.

pINA1292 plasmid map

Key features of pINA1292:

• Multi-copy integration: Allows for high levels of protein expression.

• Strong promoter (hp4d): Drives high levels of transcription.

• Signal peptide (LIP2 prepro): Directs the protein for secretion.

• Terminator (XPR2): Ensures efficient transcription termination.

• Selection marker (URA3): Allows for selection of yeast transformants.

Applications:

pINA1292 is widely used for the production of various proteins in Y. lipolytica, including enzymes, antibodies, and other therapeutic proteins. Its ability to achieve high levels of protein expression and secretion makes it a valuable tool for industrial biotechnology.

Example:

One notable example of pINA1292's use is in the overexpression of delta-12 desaturase in Y. lipolytica to increase the production of linoleic acid, a fatty acid with various health benefits.

Linoleic acid chemical structure

In this case, the gene encoding delta-12 desaturase is cloned into pINA1292, and the resulting plasmid is transformed into Y. lipolytica. The high copy number of the plasmid and the strong promoter lead to high levels of enzyme expression, resulting in increased linoleic acid production.

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