GF01 cells NTCC?表達綠色熒光蛋白的人臍靜脈內皮細胞GFP Expressing Human Umbilical Vein Endothelial Cells-BioVector NTCC Inc.
- 價 格：￥599850
- 貨 號：NTCC?-GF01C
- 產 地：北京
- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
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GF01 cells NTCC?表達綠色熒光蛋白的人臍靜脈內皮細胞GFP Expressing Human Umbilical Vein Endothelial Cells-BioVector NTCC Inc.
GFP expressing human umbilical vein endothelial cells were isolated from normal human umbilical vein and transfected with GFP-lentiviral particles at passage 1. Puromycin resistant GFP-HUVECs are passage 3 and are shipped frozen (or in proliferating culture with a confluency of >90 %, GF01-T25). ENDO-Growth Media (MED001) is recommended for culture. Cells have an average additional population doubling levels >18 when cultured.
- Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
- Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
- Cytoplasmic PECAM1 >95% positive by immunofluorescence
HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.
IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.
Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.
生產廠家Supplier:
BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心
E-mail:BioVector@163.com
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