pORI19 BioVector?乳酸乳球菌表達載體Lactococcus lactis expression plasmid

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

pORI19 is a plasmid commonly used in Lactococcus lactis, a bacterium often employed in food fermentation. It's a versatile tool for genetic engineering in these bacteria.

pORI19 plasmid map

Key Features:

• Origin of replication (Ori): Allows the plasmid to replicate within L. lactis.

• Erythromycin resistance gene (Emr): Confers resistance to the antibiotic erythromycin, enabling selection of bacteria carrying the plasmid.

• Multiple cloning site (MCS): Contains various restriction enzyme recognition sites, facilitating the insertion of foreign DNA fragments.

• lacZα gene: Encodes a portion of the β-galactosidase enzyme, used for blue-white colony screening to identify recombinant clones.

Applications:

• Gene expression: Introducing foreign genes into L. lactis for heterologous protein production.

• Metabolic engineering: Modifying the metabolic pathways of L. lactis to improve its performance in food fermentation.

• Strain improvement: Enhancing the characteristics of L. lactis strains used in dairy products.

How it Works:

1. Transformation: pORI19 is introduced into L. lactis cells through electroporation or other transformation methods.

2. Selection: Cells containing the plasmid are selected by growing them on media containing erythromycin.

3. Gene cloning: Foreign DNA fragments can be inserted into the MCS using appropriate restriction enzymes and ligases.

4. Recombinant protein expression: If a gene encoding a protein of interest is inserted into the MCS, the plasmid can drive its expression in L. lactis.

Advantages:

• Stable replication: pORI19 replicates stably in L. lactis.

• Easy selection: Erythromycin resistance simplifies the selection of transformed cells.

• Versatile cloning: The MCS allows for the insertion of various DNA fragments.

Limitations:

• Limited copy number: The copy number of pORI19 in L. lactis is relatively low, which may limit protein expression levels.

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BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

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