K562-IL15 NTCC?人慢性髓系白血病細胞IL-15穩轉細胞株Human chronic myelogenous leukemia Cell Line

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

The NTCC? K562-IL15 cell line is a genetically engineered variant of the K562 cell line. It is a valuable tool in immunology research, particularly in the field of natural killer (NK) cell biology.

K562IL15 cells

K562 Cell Line:

• Origin: Derived from a patient with chronic myelogenous leukemia (CML).

• Characteristics: Does not express MHC class I molecules, making it a suitable target for NK cell cytotoxicity assays.


K562-IL15 cells

IL-15 Transduction:

• IL-15: Interleukin-15 is a cytokine that plays a crucial role in the development, survival, and activation of NK cells.

• Transduction: K562 cells are genetically modified to express IL-15 on their surface.

Applications of K562-IL15 Cells:

1. NK Cell Expansion:

   • K562-IL15 cells are used as feeder cells to expand NK cells in vitro.

   • The membrane-bound IL-15 provides continuous stimulation to NK cells, promoting their proliferation and activation.

   • This allows for the generation of large numbers of NK cells for research and therapeutic purposes.

   
   


NK cell expansion using K562IL15 cells

2. NK Cell Functional Assays:

   • K562-IL15 cells can be used as target cells in cytotoxicity assays to evaluate the functional activity of NK cells.

   • The ability of NK cells to lyse K562-IL15 cells indicates their cytotoxic potential.

3. NK Cell-Based Immunotherapy:

   • Expanded NK cells using K562-IL15 cells can be used for adoptive cell therapy in cancer treatment.

   • NK cells are infused back into the patient to target and destroy tumor cells.

Advantages of K562-IL15 Cells:

• Efficient NK Cell Expansion: K562-IL15 cells provide robust and consistent stimulation for NK cell expansion.

• Versatility: Suitable for various research and clinical applications.

Limitations:

• Potential for Contamination: K562 cells can contaminate NK cell cultures if not properly handled.

• Long-Term Culture: Prolonged culture of K562-IL15 cells may lead to changes in their properties.

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BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

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