Clostridium novyi VPI 8099 NTCC?諾維氏梭菌菌株27606 BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

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NTCC? Clostridium novyi VPI 8099諾維氏梭菌菌株27606

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

NTCC? Clostridium novyi VPI 8099 (ID No: 27606) is a specific strain of the bacterium Clostridium novyi, a type A strain.

Clostridium novyi: This bacterium is a common inhabitant of soil and the intestines of animals. It is known for its ability to cause gas gangrene, a severe and potentially life-threatening infection.
VPI 8099: This designation likely refers to the specific source or origin of this particular strain. "VPI" may stand for the Virginia Polytechnic Institute and State University, suggesting that this strain was isolated or characterized at that institution.

Key Points:

Gas Gangrene: Clostridium novyi, including strain VPI 8099, can produce toxins that cause rapid tissue destruction, leading to gas gangrene.
Serious Infection: Gas gangrene is a medical emergency requiring immediate treatment, often involving antibiotics, surgery, and potentially hyperbaric oxygen therapy.
Strain Specificity: While the general characteristics of Clostridium novyi are well-understood, specific strains like VPI 8099 may exhibit slight variations in their behavior or virulence.

2b. Clostridium novyi Review (part 2) | HIPRA

Clostridium novyi - an overview | ScienceDirect Topics

Medium: NTCC? Medium 1589: Modified chopped meat medium (NTCC? medium 1490) with 1% glucose
Temperature: 37°C
Atmosphere: Anaerobic
Handling procedure: 1. Open vial according to enclosed instructions.

2. Under anaerobic conditions, withdraw 0.5 ml of the recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
5. Within 48 hours, growth should be evident by turbidity and gas in the broth. No growth should occur on agar plate incubated aerobically. After good growth has been established in broth, an agar plate may be streaked and incubated anaerobically to observe colonial morphology.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
? Use of an anaerobic gas chamber, or
? Placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
? Loose screw caps on test tubes in anaerobic chamber,
? Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
? Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes: Minimize exposure to oxygen, or strain will not remain viable. Thioglycollate medium does not support growth. Use only pre-reduced media that has been stored anaerobically.

Cells are large regular rods. Spores are formed subterminally. Colonies are irregular with a lobate margin.