pcDNA3.1-2xFLAGSREBP-2 BioVector?人源固醇調節元件結合蛋白表達質粒

BioVector NTCC質粒載體菌株細胞蛋白抗體基因保藏中心

pcDNA3.1-2xFLAGSREBP-2 is a plasmid vector designed for expressing the Sterol Regulatory Element Binding Protein 2 (SREBP-2) protein in mammalian cells.

Key Features:

• pcDNA3.1 Vector: This is a widely used mammalian expression vector. It contains a strong promoter (CMV promoter) that drives high levels of gene expression in mammalian cells.

• 2xFLAG-Tag: The SREBP-2 protein is fused with two copies of the FLAG epitope. This tag allows for easy detection and purification of the expressed protein using anti-FLAG antibodies.

• SREBP-2 Gene: This gene encodes SREBP-2, a transcription factor that plays a crucial role in regulating cholesterol and lipid metabolism.

Applications:

• Overexpression of SREBP-2: This plasmid can be used to overexpress SREBP-2 in cells to study its effects on gene expression and cellular function.

• Protein Purification: The FLAG-tag facilitates the purification of the expressed SREBP-2 protein using anti-FLAG affinity chromatography.

• Drug Discovery: This plasmid can be used to screen for compounds that modulate SREBP-2 activity and may have therapeutic potential for treating metabolic disorders.

Note:

• The use of pcDNA3.1-2xFLAGSREBP-2 requires appropriate safety measures in the laboratory, as it involves working with genetically modified organisms.

• The expression and activity of SREBP-2 can be influenced by various factors, such as cell type, culture conditions, and the presence of other cellular proteins.

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