BHK/T7-9 NTCC?穩(wěn)定表達(dá)T7 RNA聚合酶的倉鼠腎源性BHK細(xì)胞cell line-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
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- 貨 號:BHK/T7-9 NTCC?穩(wěn)定表達(dá)T7 RNA聚合酶的倉鼠腎源性BHK細(xì)胞cell line-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
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BHK/T7-9 NTCC?穩(wěn)定表達(dá)T7 RNA聚合酶的倉鼠腎源性BHK細(xì)胞cell line-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心
Comment | A hamster kidney-derived BHK cell clone stably expressing T7 RNA polymerase. |
Year of deposit | 2018 | ||||
Animal | _hamster < Mammals | ||||
Strain name | Syrian | ||||
Genus | Mesocricetus | ||||
Species | auratus | ||||
Gender | Unknown | ||||
Tissue | kidney | ||||
Classification | other | ||||
Recombinant | recombinant | ||||
Exogene | T7 RNA polymerase gene | ||||
2.9K base | |||||
Vector | pCAGGS/MCS | ||||
Selection | 600 μg/ml hygromycin (every 10 passages) | ||||
Lifespan | infinite | ||||
Morphology | epithelial-like | ||||
Cellosaurus(Expasy) | CVCL_A8V7 | ||||
Medium | Medium List | ||||
Culture type | Adherent cells | Adherent cells | |||
Culture medium | Eagle’s MEM with 5% Fetal Calf Serum and 10% Tryptose Phosphate Broth (BD, Difco 260300) | MEM + 5% FBS + 10% Tryptose Phosphate Broth ( Media preparation ) | |||
Antibiotics | 100 U/ml penicillin and 100 U/ml streptomycin | Free | |||
Passage method | Trypsin-EDTA | 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA) | |||
Designated culture dish | 100 mm dish | ||||
Culture information | Passage ratio | 1:5 – 1:10 | 1 : 8 split | ||
SC frequency | Subculture : once/4 days | Subculture : once/4 days | |||
Temperature | 37 ℃ | 37 ℃ | |||
CO2 concentration | 5 % | 5 % | |||
Freeze medium | CELLBANKER 1 | CELLBANKER 1 | |||
Freezing method | Slow freezing | ||||
Mycoplasma/Acholeplasma | (-) | ||||
Mycoplasma | (-) | ||||
Animal PCR | OK | ||||
Images | |||||
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