pCMV-Myc-UCP1 BioVector?基因UCP1過表達(dá)質(zhì)粒mammalian expression plasmid

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

The pCMV-Myc-UCP1 plasmid is a valuable tool for expressing the uncoupling protein 1 (UCP1) protein in mammalian cells. Here's a breakdown of its key features and significance:

Key Features:

• Promoter: The plasmid utilizes the strong CMV (Cytomegalovirus) promoter, which drives high levels of gene expression in a wide range of mammalian cell types.

• Myc-Tag: The UCP1 gene is fused to the Myc epitope tag at its N-terminus. This tag allows for easy detection and purification of the expressed UCP1 protein using anti-Myc antibodies.

• Ampicillin Resistance: The plasmid carries the ampicillin resistance gene, enabling selection of bacterial colonies harboring the plasmid during propagation in E. coli.

• Mammalian Expression Vector: The plasmid is designed specifically for expression in mammalian cells, ensuring compatibility with the cellular machinery.

Significance:

• UCP1 Research: UCP1 is a key protein involved in thermogenesis, particularly in brown adipose tissue. This plasmid facilitates the study of UCP1 function, regulation, and its role in energy metabolism.

• Drug Discovery: By expressing UCP1 in various cell models, researchers can investigate potential therapeutic targets for obesity and metabolic disorders.

• Protein Production: The plasmid enables the production of recombinant UCP1 protein for biochemical and structural studies.

Visual Representation:

Applications:

• Investigating UCP1's role in thermogenesis and energy expenditure.

• Screening for compounds that modulate UCP1 activity.

• Studying the interaction of UCP1 with other proteins.

• Producing recombinant UCP1 protein for further analysis.

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BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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