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載體基本信息
出品公司: | -- |
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載體名稱: | pRI101 |
質粒類型: | 植物表達載體 |
高拷貝/低拷貝: | -- |
啟動子: | -- |
克隆方法: | 多克隆位點,限制性內切酶 |
載體大小: | -- |
5' 測序引物及序列: | -- |
3' 測序引物及序列: | -- |
載體標簽: | -- |
載體抗性: | -- |
篩選標記: | -- |
備注: | -- |
產品目錄號: | -- |
穩定性: | -- |
組成型: | -- |
病毒/非病毒: | -- |
載體質粒圖譜和多克隆位點信息
載體簡介
pRI 101 DNA vectors are binary plant transformation vectors intended for foreign gene expression in plant cells. These agrobacterium-mediated plant transformation vectors carry the 35S promoter of caμliflower mosaic virus (CaMV) and the 5’ non-coding region (5’-UTR) of the alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translation enhancer in plants. There are two types of pRI 101 DNA vectors, the pRI 101-AN DNA vector, which carries the 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR), and the pRI 101 ON DNA vector, which carries the 5’-UTR of rice ADH (OsADH 5’-UTR). The pRI 101-AN DNA vector is for dicotyledonous plants such as tobacco or Arabidopsis while the pRI 101-ON DNA vector is for monocotyledonous plants such as rice.FeaturesAgrobacterium-mediated plant transformationEasy and stable integration of dicot and monocot target genes into plant chromosomesIncreased target gene expression via the 5'-UTR translation enhancer region Vector versatility for dicotyledonous and monoctyledonous plant cells
載體序列
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