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載體基本信息
| 出品公司: | |
|---|---|
| 載體名稱: | pBI121 |
| 質粒類型: | 植物雙元表達載體 |
| 高拷貝/低拷貝: | 高拷貝 |
| 啟動子: | CaMV35S |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 14758 bp |
| 5' 測序引物及序列: | 35S Promoter: CTATCCTTCGCAAGACCCTTC |
| 3' 測序引物及序列: | -- |
| 載體標簽: | C-Gus |
| 載體抗性: | 卡那 |
| 篩選標記: | Beta-Glucuronidase |
| 備注: | 雙元植物表達載體，帶有Gus報告基因，方便對轉染植物進行篩選。 |
| 產品目錄號: | -- |
| 穩定性: | 穩定 |
| 組成型: | 組成型 |
| 病毒/非病毒: | 非病毒 |
載體質粒圖譜和多克隆位點信息
載體簡介
pBI121載體是一個雙元植物農桿菌表達載體，能夠高效轉染植物。該載體來源于pB221和Bin19載體。載體上含有從ColE1來源的ori復制元件，從CaMV中來源的pROK1元件，大腸桿菌新霉素磷酸轉移酶II基因（Neomycin phosphotransferase II Gene, NptII），新霉素抗性基因(Neomycin resistant gene, Neo), 敏感基因Beta葡糖苷酸酶(glucuronidase)，Ter 農桿菌Ti質粒胭脂氨酸合成酶原件等。pBI121載體是卡那抗性質粒。
載體序列
LOCUS pBI121 14758 bp ds-DNA circular SYN 21-April-2013
DEFINITION Binary Agrobacterium vector with a GUS reporter gene for plant
transformation.
ACCESSION AF485783
VERSION .
KEYWORDS pBI121
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14758)
AUTHORS Chen PY, Wang CK, Soong SC, To KY.
TITLE Complete sequence of the binary vector pBI121 and its application in
cloning T-DNA insertion from transgenic plants.
JOURNAL Mol. Breed. 11, 287-293 (2003)
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..14758
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS complement(145..516)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label="traJ"
oriT 549..658
/label="incP origin of transfer"
CDS complement(1339..1989)
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/label="TetR"
misc_feature 2454..2478
/label="RB T-DNA repeat"
/label="right border repeat from nopaline C58 T-DNA"
promoter 2602..2781
/label="NOS promoter"
/label="nopaline synthase promoter"
CDS 2838..3632
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label="NeoR/KanR"
/label="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
terminator 4022..4274
/label="NOS terminator"
/label="nopaline synthase terminator and poly(A) signal"
promoter 4859..4889
/label="lac promoter"
/label="promoter for the E. coli lac operon"
protein_bind 4897..4913
/bound_moiety="lac repressor encoded by lacI"
/label="lac operator"
/label="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4921..4937
/label="M13 rev"
/label="common sequencing primer, one of multiple similar
variants"
promoter 5461..5806
/label="CaMV 35S promoter"
/label="strong constitutive promoter from cauliflower mosaic
virus"
CDS 5845..7656
/codon_start=1
/gene="uidA?(or gusA)?from E. coli"
/product="beta-glucuronidase"
/label="GUS"
terminator 7727..7979
/label="NOS terminator"
/label="nopaline synthase terminator and poly(A) signal"
primer_bind complement(7988..8004)
/label="M13 fwd"
/label="common sequencing primer, one of multiple similar
variants"
misc_feature 8622..8646
/label="LB T-DNA repeat"
/label="left border repeat from nopaline C58 T-DNA"
CDS complement(10242..11390)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label="trfA"
CDS complement(11689..12483)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label="KanR"
/label="confers resistance to kanamycin"
mobile_element 12675..13442
/mobile_element_type="insertion sequence:IS1"
/label="IS1"
/label="prokaryotic transposable element"
rep_origin 14065..18
/label="oriV"
/label="incP origin of replication"
ORIGIN
1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaat您正在向 biovector.net 發送關于產品 pBI121質粒載體圖譜序列抗性價格報價Biovector NTCC Inc.現貨 的詢問
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