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BACKBONE
- Vector backbonepT7TS (based on pGEM4Z)
Backbone size (bp)3000
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)DH5alpha
- Copy numberUnknown
SEQUENCE INFORMATION
Depositor Sequences
None.
Full plasmid sequence is available only if provided by the depositing laboratory
.
GENE/INSERT
- Gene/Insert nameNone
- GenBank IDAB255037
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ sequencing primerT7
3′ sequencing primerSP6
RESOURCE INFORMATION
- DEPOSITOR COMMENTS
5' & 3' untranslated regions of Xenopus Beta-globin mRNA cloned into pGEM4Z. Insert size: 260 bp. Transcription: transcribe with T7 RNA polymerase. Cloning sites: Bgl II, EcoRV, SpeI
Xenbase has a nice description of this vector: http://www.xenbase.org/reagents/vectorAction.do?method=displayVectorSummary&vectorId=11536497&tabId=0
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