LOCUS       Exported                4726 bp ds-DNA     circular SYN 12-AUG-2016
KEYWORDS    pCMV-RFP-C-Myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Biofeng Lab.
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        67..370
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..618
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        625..643
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    650..652
                     /note="ATG"
     misc_feature    659..696
                     /note="MCS"
     gene            707..1399
                     /note="RFP"
     CDS             1400..1429
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     polyA_signal    1459..1584
                     /note="bGH poly(A)"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1642..2097)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2124..2228
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        2328..2587
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      2438..2573
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             2622..3416
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3648..3695
                     /note="HSV TK poly(A)"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4001..4644
                     /direction=RIGHT
                     /note="pUC ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tcccgggagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      601 ttactggctt atcgaaattc tagataatac gactcactat agggcgaata tggccacggg
      661 cgcgccctgc aggctagcct cgagggatcc gatatcgcgg ccgcttcttt ccaaacaagt
      721 tttgcctcat gatgtgaaaa tgagatacca tatggatgga tgtgttaacg gtcacagttt
      781 cactattgag ggagaaggtg ctggtaaacc ttatgaaggc aaaaagatct tggaactgcg
      841 tgtaacaaag ggaggaccac ttccattcgc ctttgacatt ttgtccagcg ttttcactta
      901 tggtaacagg tgtttctgtg agtatccaga ggatatgccc gactacttta agcaatcatt
      961 gcccgagggt cactcttggg agagaacttt aatgtttgag gatggaggat gcggtactgc
     1021 ttcagcccat atatcgttgg acaaaaactg tttcgtgcat aagtctacgt ttcatggtgt
     1081 gaattttcct gctaatggtc cggtcatgca gaaaaaggca atgaattggg agcctagctc
     1141 tgaactgatc accgcatgtg atggtattct caaaggcgac gttaccatgt ttttgttgct
     1201 ggaaggtggt cacagattaa agtgccaatt cacaacatcc tacaaggctc ataaagcagt
     1261 gaagatgcca ccaaatcaca ttatcgagca tgtcctagtc aaaaaggaag ttgctgacgg
     1321 ttttcaaatc caggaacacg ctgttgcaaa acacttcaca gtagatgtta aggaaactcg
     1381 cggccgcgaa ttcagatctg agcagaagct gatctccgag gaggacctgg ttaacttatg
     1441 ataaggccgg cctgatcaaa aatcagcctc gactgtgcct tctagttgcc agccatctgt
     1501 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     1561 ctaataaaat gaggaaattg catctgtaca tctagcattc agttgtggtt tgtccaaact
     1621 catcaattta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1861 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1921 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1981 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     2041 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2221 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     2281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     2461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     2521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     2581 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     2641 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     2701 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     2761 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     2821 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     2881 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     2941 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     3001 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     3061 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     3121 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     3181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     3241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     3301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     3361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     3421 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     3481 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     3541 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     3601 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     3661 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     3721 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     3781 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     3841 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4141 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//