HaCaT Cells

BioVector NTCC Inc.

Origin:

In vitrospontaneously transformed keratinocytes from histologically normal skin.

Species:

Homo sapiens

Tissue:

Skin

Morphology:

Keratinocyte

Properties:

Adherent monolayer

Cytogenicdata:

Patient:

Male, Caucasian, 62 years of age.

Medium:

DMEM + 10% FBS

Subculture:

Recommended 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C

FreezingMedium:

Complete culture medium supplemented with 5% (v/v) DMSO

BiosafetyLevel:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

DNAProfile (STR):

Amelogenin: X,X

CSF1PO: 9,11

D13S317: 10,12

D16S539: 9,12

D5S818: 12

D7S820: 9,11

THO1: 9.3

TPOX: 11,12

vWA: 16,17

HaCaTCells References:

1. Nakai, K., Yoneda, K., et al. HB-EGF-induced VEGF production and eNOSactivation depend on both PI3 kinase and MAP kinase in HaCaT cells. J DermatolSci, 55: 170-178, 2009.

2. Markopoulou, S., Kontargiris, E., Batsi, C., Tzavaras, T., Trougakos,I., Boothman, D. A., Gonos, E. S., and Kolettas, E. Vanadium-induced apoptosisof HaCaT cells is mediated by c-fos and involves nuclear accumulation ofclusterin. Febs J, 276: 3784-3799, 2009.

3. Aksoy P, et al. HPV16 infection of HaCaTs is dependent on β4integrin,and α6 integrin processing. Virology, Jan 20;449:45-52, 2014.PMID: 24418536

4. Mohammad H, Cushman M, Seleem MN. Antibacterial Evaluation of SyntheticThiazole Compounds In Vitro and In Vivo in aMethicillin-ResistantStaphylococcus aureus (MRSA) Skin Infection Mouse Model.PLoS One. 2015 Nov 4;10(11):e0142321.PMID: 26536129

5. Aijaz A, Faulknor R, Berthiaume F, Olabisi RM.HydrogelMicroencapsulated Insulin-Secreting Cells Increase Keratinocyte Migration,Epidermal Thickness,Collagen Fiber Density, and Wound Closure in a DiabeticMouse Model of Wound Healing. Tissue Eng Part A. 2015 Nov;21(21-22):2723-2732.PMID: 26239745

6. Crochiere M, Kashyap T, Kalid O, Shechter S, Klebanov B, Senapedis W,Saint-Martin JR, Landesman Y. Deciphering mechanisms of drug sensitivity andresistance to Selective Inhibitor of Nuclear Export (SINE)compounds. BMCCancer. 2015 Nov 17;15:910.PMID: 26573568