pHIV-Luc-ZsGreen慢病毒雙熒光表達(dá)載體質(zhì)粒-BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
- 價 格：￥7930
- 貨 號：pHIV-Luc-ZsGreen plasmid
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
- 已注冊
pHIV-Luc-ZsGreen
Plasmid 39196
-BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
描述：
pHIV-Luc-ZsGreen慢病毒雙熒光表達(dá)載體質(zhì)粒，螢火蟲酶熒光和ZsGreen綠色熒光通過IRES雙順反子元件連接雙表達(dá)
- Vector backbonepHIV-Zsgreen(Search Vector Database)
Total vector size (bp)9312
- Modifications to backboneThis plasmid was generated by cloning Luc2P (firefly luciferase) into the EcoRI and XbaI sites of pHIV-ZsGreen (Addgene Plasmid 18121).
- Vector typeMammalian Expression, Lentiviral
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Copy numberHigh Copy
GENE/INSERT 1
- Gene/Insert nameFirefly Luciferase Luc2P
- Insert Size (bp)1600
PromoterEF1-alpha
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodRestriction Enzyme
5′ cloning siteEcoRI (not destroyed)
3′ cloning siteXbaI (not destroyed)
5′ sequencing primerEF1a Fwd Primer
GENE/INSERT 2
- Gene/Insert nameZsGreen
- Insert Size (bp)700
PromoterEF1-alpha
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodRestriction Enzyme
5′ cloning siteMscI (not destroyed)
3′ cloning siteClaI (not destroyed)
5′ sequencing primern/a
3′ sequencing primerpBluescriptKS Primer
>Full plasmid sequence
gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcata
gttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagct
acaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttc
gcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggg
gtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctga
ccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactt
tccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat
gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgacc
ttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggtttt
ggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgt
caatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattg
acgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtct
ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaat
aaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct
cagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaaggga
aaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcg
actggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagt
attaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata
aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaac
atcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttaga
tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaag
ctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgcgctga
tcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaa
aattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtg
ggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgc
tgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattga
ggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtg
gaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactg
ctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatgga
gtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaa
gaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaa
attggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgc
tgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaacc
ccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattc
gattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaatttta
aaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacataca
aactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagat
ccagtttggttagtaccgggcccgctctagccgtgaggctccggtgcccgtcagtgggcagagcgcacat
cgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcgg
ggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatata
agtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgt
gtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggct
gcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa
ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggt
ggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgc
gacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggttttt
ggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgc
ggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgcc
gtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg
cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcac
ccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgcc
gtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttat
gcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattct
ccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttt
ttttcttccatttcaggtgtcgtgagcggccgctgaactgAATTCATGgaagatgccaaaaacattaaga
agggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcg
ctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgag
tacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcg
tggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgt
ggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtc
gtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaaga
tcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccattt
gccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatc
atgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgat
tcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgcc
atttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatg
taccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgc
ccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagat
cgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggc
atccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagc
ctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacact
gggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaacccc
gaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgagg
acgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagc
cgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgac
gatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcg
tggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggt
gcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggc
ggcaagatcgccgtgtaataattctagagtacccgggctaggatccgcccctctccctccccccccccta
acgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatatt
gccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctt
tcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttctt
gaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctg
cggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttgg
atagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaagg
taccccattgtatgggatctgatctggggcctcggtAcacatgctttacatgtgtttagtcgaggttaaa
aaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataatatggccac
aaccatggcccagtccaagcacggcctgaccaaggagatgaccatgaagtaccgcatggagggctgcgtg
gacggccacaagttcgtgatcaccggcgagggcatcggctaccccttcaagggcaagcaggccatcaacc
tgtgcgtggtggagggcggccccttgcccttcgccgaggacatcttgtccgccgccttcatgtacggcaa
ccgcgtgttcaccgagtacccccaggacatcgtcgactacttcaagaactcctgccccgccggctacacc
tgggaccgctccttcctgttcgaggacggcgccgtgtgcatctgcaacgccgacatcaccgtgagcgtgg
aggagaactgcatgtaccacgagtccaagttctacggcgtgaacttccccgccgacggccccgtgatgaa
gaagatgaccgacaactgggagccctcctgcgagaagatcatccccgtgcccaagcagggcatcttgaag
ggcgacgtgagcatgtacctgctgctgaaggacggtggccgcttgcgctgccagttcgacaccgtgtaca
aggccaagtccgtgccccgcaagatgcccgactggcacttcatccagcacaagctgacccgcgaggaccg
cagcgacgccaagaaccagaagtggcacctgaccgagcacgccatcgcctccggctccgccttgccctaa
ggatcgcatcgataccgtcgacctcgatcgagacctagaaaaacatggagcaatcacaagtagcaataca
gcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacac
ctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggg
gggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaa
ggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggt
gctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgtt
acaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgacagccgc
ctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatct
gagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgct
tcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtg
tggaaaatctctagcagcatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgc
tggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcg
aaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg
accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac
gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca
gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcca
ctggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagt
ggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacctt
cggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc
aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacg
ctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagat
ccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttac
caatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactcc
ccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgaga
cccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggt
cctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccag
ttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggc
ttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtt
agctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag
cactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaa
gtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcg
ccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct
taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttacttt
caccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgg
aaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatga
gcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagt
gccacctgac
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