IPEC-J2 Cell Line豬小腸上皮細胞株

-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

Cat No.: NTCC510701

Cell line:

IPEC-J2

Species:

porcine (pig) (Sus scrofa)

Cell type:

intestine (jejunum)

Origin:

established from normal intestinal epithelium cells isolated from the jejunum of a neonatal, unsuckled pig

Biosafety level:

1

Permissions and restrictions:

A

Cell Culture Data:

Morphology:

epitheloid cells growing adherently as monolayer;

Medium:

90% Dulbecco′s MEM (4.5 g/L glucose) + 10% h.i. FBS

Subculture:

seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA

Incubation:

at 37 °C with 5% CO2

Doubling time:

ca. 40-60 hours

Harvest:

cell harvest of ca. 8 x 106 cells/80 cm2

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Species:

confirmed as pig by species PCR