K-562/Adr Cell Line人白血病細胞阿霉素耐藥株

Catalog Number: NTCC528571

BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

Growth Properties : suspension

Organism : Homo sapiens (human)

Source : Organ: bone marrow

Cell type: lymphoblast

Disease: chronic myelogenous leukemia (CML)

Comments : The K-562 cell line has attained widespread use as a highly sensitive in vitro target for the

natural killer assay.

Drug resistance: The highest concentration of adriamycin resistance is 1000ng/ml.

Propagation : Complete growth medium: DMEM, 90%; fetal bovine serum, 10%

Temperature: 37.0℃,5%CO2

Subculturing:

Protocol: Cultures can be maintained by the addition or replacement of fresh medium. Start

new cultures at 1 x 105 viable cells/mL. Subculture at 1 x 106 cells/mL.

Medium renewal: Every 2 to 3 days

Preservation : Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: Liquid nitrogen vapor temperature