E.coli DH10B T1 Phage-Resistant strain, competent cells

E.coli DH10B T1R

-BioVector NTCC Inc.

Features of DH10B T1 Phage-Resistant strain

? Permit efficient cloning of methylated DNA

? Are resistant to T1 and T5 bacteriophages

? Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal

? Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation

The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable

libraries against contamination. Additionally, mutations in the methylation-dependent restriction system

(mcrA, mcrBC, and mrr) make DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of

both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes.

DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA

libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the

Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or

Bluo-Gal. Finally, theendA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for

subsequent extraction and purification.

Genotype:

F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15

ΔlacX74 recA1 endA1araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG tonA