pOET1昆蟲桿狀病毒表達載體-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

Plasmid Description:

pOET1 is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful

AcMNPV polyhedron (polh) promoter. The vector is smaller than other available transfer vectors (4541bp)

which greatly facilitate the cloning steps. It has a bacterial origin of replication and an ampicillin resistance

gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site containing 14

unique restriction sites for insertion of the foreign gene in the correct orientation. The AcMNPV sequences

flanking the gene in the transfer vectors MCS allow recombination with the viral DNA to insert the expression

cassette into the polh locus. pOET1 is compatible with any baculovirus system that utilizes homologous

recombination in insect cells.

Sequence序列：

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