pOET2昆蟲桿狀病毒表達載體-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

Plasmid Description:

pOET2 is a baculovirus transfer vector designed for high level expression of foreign genes under the powerfulAcMNPV polyhedron (polh) promoter. The vector is smaller than other available transfer vectors (4547bp)which greatly facilitate the cloning steps. It has a bacterial origin of replication and an ampicillin resistance genefor selection in E. coli. The polh sequences have been replaced by a multiple cloning site in the reverseorientation to pOET1, containing unique restriction sites for insertion of the foreign gene. The AcMNPVsequences flanking the gene in the transfer vectors MCS allow recombination with the viral DNA to insert theexpression cassette into the polh locus. pOET2 is compatible with any baculovirus system that utilizeshomologous recombination in insect cells

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