pOET4昆蟲桿狀病毒表達載體-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

Plasmid Description:

pOET4 is a baculovirus transfer vector designed for high level expression of foreign genes under the lateAcMNPV basic (p6.9) promoter. Using this promoter will provide earlier expression compared to thepolyhedrin promoter. This has been shown to be beneficial when expressing proteins which require extensivepost-translational modifications i.e. glycosylation. The vector is smaller than other available transfer vectors(4536bp) which greatly facilitate the cloning steps. It has a bacterial origin of replication and an ampicillinresistance gene for selection in E. coli. The AcMNPV sequences flanking the gene in the transfer vectors MCSallow recombination with the viral DNA to insert the expression cassette into the polyhedrin locus. Thepolyhedrin sequences have been replaced by a multiple cloning site containing unique restriction sites forinsertion of the foreign gene in the correct orientation. pOET4 is compatible with any baculovirus system thatutilizes homologous recombination in insect cells.

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