Organism

Mus musculus, mouse

Tissue

intestine

Cell Type

intestinal neuroendocrine tumor      cells

Product Format

frozen 1.0 mL

Morphology

epithelial-like

Culture Properties

adherent

Biosafety Level

1

Disease

invasive small intestinal      neuroendocrine carcinoma

Age

10 to 13 weeks

Strain

C57B1/6J

Applications

This cell line may be a useful      model for human neuroendocrine neoplasms of the gut, and useful tools for      studying hormone secretin.

Derivation

The STC-1 cell line was derived      from the intestinal tumors of RIP1Tag2/Rip2pyST1 double transgenic mice.

Cellular Products

secretin

Comments

The STC-1 cell line was derived      from the intestinal tumors of double transgenic mice. Transgenic mice harboring      a hybrid gene linking the rat insulin promoter (RIP) to polyoma small T      (PyST) antigen were mated with transgenic mice harboring rat insulin promoter      (RIP) linked to SV40 early region (Tag) creating off-spring harboring both      transgenes (double transgenics). These mice were found to have frequent      intestinal tumors in addition to pancreatic Beta-cell tumors. Gene expression      studies suggested that the intestinal and pancreatic tumors arose as separate      entities. The STC-1cell line produces the hormone secretin. This cell line      may be a useful model for human endocrine neoplasms of the gut.

Complete Growth Medium

The base medium for this cell line      is NTCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.      To make the complete growth medium, add the following components to the base      medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cells must be subcultured when they      reach ~70% confluence, or else they start to come off the flask into      suspension.
 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or      increase amount of dissociation medium for culture vessels of other      sizes.

1.Remove and discard culture medium. Briefly rinse the      cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (NTCC 30-2200) or      0.05% Trypsin – 0.02% EDTA (NTCC PCS-999-003) solution to remove      all traces of serum which contains trypsin inhibitor.

2.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and      observe cells under an inverted microscope until cell layer is dispersed      (usually within 5 to 15 minutes).
 Note: To avoid clumping do not agitate the cells by hitting or shaking      the flask while waiting for the cells to detach. Cells that are difficult to      detach may be placed at 37°C to facilitate dispersal.

3.Add 6.0 to 8.0 ml of complete growth medium and      aspirate cells by gently pipetting.

4.Transfer cell suspension to a centrifuge tube and spin      at approximately 125 xg for 5 to 10 minutes.

5.Discard supernatant. Resuspend the cell pellet in fresh      growth medium.

6.Add appropriate aliquots of the cell suspension to new      culture vessels. Incubate cultures at 37°C.

Subcultivation Ratio: 1:3 to      1:5 is recommended.
 Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth      medium supplemented with 10% (v/v) DMSO
 Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C
 Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Year of Origin

June 1990

References

Rindi G, et al. Development of      Neuroendocrine Tumors in the Gastrointestinal Tract of Transgenic Mice. Am J      Pathol 136(6): 1349-1363, 1990. PubMed: 2162628

Grant SGN, et al. Early      Invasiveness Characterizes Metastatic Carcinoid Tumors in Transgenic Mice.      Cancer Res 51: 4917-4923, 1991. PubMed: 1654206