L4440-GFP線蟲基因沉默干擾載體熒光標記質粒

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Map圖譜

Full-length GFP sequence (from vector p95.77) was cloned into RNAi feeding vector L4440. Use in a strain carrying a GFP construct to observe reduction of GFP signal after RNAi induction.

Vector backbone L4440 (Search Vector Database)

Backbone size w/o

insert (bp) 2790

Vector type Worm Expression, RNAi

GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin

Growth Temperature 37°C

Growth Strain(s) DH5alpha

Copy number High Copy

GENE/INSERT

Gene/Insert name GFP

Alt name green fluorescence protein

Insert Size (bp) 869

CLONING INFORMATION

Cloning method Restriction Enzyme

5′ sequencing primer L4440 primer (AGCGAGTCAGTGAGCGAG)