E.coli BL21-CodonPlus(DE3)-RIPL大腸桿菌表達宿主菌

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Antibiotic Resistance

E.coli BL21-CodonPlus(DE3)-RIPL strain is streptomycin, tetracycline, and chloramphenicol resistant.

GenotypeE. coli B F- ompT hsdS(rB- mB-) dcm+ Tetr gal λ(DE3) endA Hte [argU proLCamr] [argU ileY leuW Strep/Specr]

Description

BL21-CodonPlus(DE3)-RIPLstrain produces an increased supply of rare E. coli tRNAs that correspond tocodons used more frequently by other organisms. This novel host contains extracopies of argU, ileW, leuY and proL tRNAs that recognize rare arginine,isoleucine, leucine and proline codons, respectively. Use this strain toovercome expression problems due to codon bias.