pCCFP-X植物雙分子熒光互補載體

BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

www.biovector.net

BiFC System info:

This Bimolecular Fluorescence Complementation system is based on the 172

N-terminal amino acids of EYFP, and the 85 C-terminal aa.s of ECFP (aa.s 154-239).

Use of the 4 different vectors yields C- and N-terminal fusions of a putative interactor

protein to both of the of the fluorescent protein halves.

All 4 vectors are binary with a backbone derived from pPZP312, a single 35S promoter

and a CaMV terminator (derived from pRT100).

The vectors confer Spectinomycin resistance in E. coli and Agrobacterium, and Basta

resistance in plants.

All vectors contain a 6xHis-tag allowing for simple affinity-based purification of the

expressed proteins.

Map圖譜