pNXGW植物BiFC雙分子熒光互補載體

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35S啟動子，目的基因N端帶有YFP 1-172片段，6xHis標(biāo)簽，Gayeway技術(shù)兼容。

Map圖譜

BiFC System info:

This Bimolecular Fluorescence Complementation system is based on the 172

N-terminal amino acids of EYFP, and the 85 C-terminal aa.s of ECFP (aa.s 154-239).

Use of the 4 different vectors yields C- and N-terminal fusions of a putative interactor

protein to both of the of the fluorescent protein halves.

All 4 vectors are binary with a backbone derived from pPZP312, a single 35S promoter

and a CaMV terminator (derived from pRT100).

The vectors confer Spectinomycin resistance in E. coli and Agrobacterium, and Basta

resistance in plants.

All vectors contain a 6xHis-tag allowing for simple affinity-based purification of the

expressed proteins.