Cell Line Name:

ATDC5

Keywords:

Mouse 129  teratocarcinoma AT805 derived

Cell Line Description:

The chondrogenic  cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a  sequential transition of phenotype in vitro. They encompass stages from  mesenchymal condensation to calcification. Bone morphogenetic protein-2  (BMP-2) stimulated the sequential progression of early- and late-phase  differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide  (PTHrP) receptor resulted in inhibition of this differentiation capacity.

Species:

Mouse

Tissue of Origin:

Unknown

CellType:

Epithelial-like

Growth Mode:

Adherent

Karyotype:

Not specified

Subculture Routine:

Split sub-confluent  cultures (70-80%) i.e. seeding at 1-3 x10,000 cells/cm2 using 0.25% trypsin  or trypsin/EDTA;CO2; 37°C subculture every 2-3 days.

Culture Medium:

DMEM: Ham's F12  (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Country:

Japan

References:

Exp. Cell Res 1998;  241:1, J Bone Miner Res 1997;12:1174