Stbl4 E.coli strain, Chemically Competent Cells

Stbl4大腸桿菌菌株，化學感受態細胞

BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心www.biovector.net

產品套裝編號：NTCC161186

儲存條件：-80℃保存保存時間：12個月

Stbl4? cells are E. coli cells which can be transformed by

electroporation (1,2). These cells can only be transformed by

electroporation and are not transformed by "heat shock". These cells are

suitable for the generation of cDNA libraries using plasmid-derived

vectors. The lacZΔM15 marker provides α-complementation of the

β-galactosidase gene from pUC or similar vectors and therefore can be

used for blue/white screening of colonies on agar plates containing Xgal

or Bluo-gal and IPTG. The mcrA mutation and the mcrBC-hsdRMSmrr

deletion allow cloning of genomic sequences which are methylated.

Stbl4? is a derivative of Stbl2? (3) and is suitable for the cloning of

unstable inserts such as retroviral sequences or direct repeats (3,4,5).

Unlike Stbl2?, Stbl4? is lon+. For optimal performance, expression in

S.O.C. Medium as well as incubation on antibiotic plates should be

done at 30°C*. Stbl4? cells are capable of being transformed efficiently

with large plasmids and can also serve as a host for M13mp cloning

vectors (see Note 1).

*To maximize stabilization of direct repeats and retroviral sequences,

incubate Stbl4?cells and perform expression studies at 30°C.

Genotype

mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal- thi-1 supE44 λ- relA1

Δ(lac-proAB)/F′ proAB+ lacIqZΔM15 Tn10 (TetR)