LOCUS       Exported                4139 bp ds-DNA     circular SYN 31-JUL-2016
COMMENT     This file is created by Vector NTI
            http://www.biovector.net/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|www.biovector.net|
COMMENT     VNTNAME|pCS2+8|
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    79..165
                     /note="MCS1"
                     /note="color: #00ccff"
     promoter        complement(167..184)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    189..323
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    390..423
                     /note="MCS2"
                     /note="color: #00ccff"
     promoter        complement(436..454)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(477..479)
                     /note="ATG"
                     /note="color: #ff0000; direction: LEFT"
     protein_bind    499..515
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(523..553)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 523 .. 529 / #ffffff / -10
                        2: 530 .. 547 / #ffffff
                        3: 548 .. 553 / #ffffff / -35"
     protein_bind    568..589
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(877..1465)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(1636..2496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1636 .. 2427 / #ccffcc
                        2: 2428 .. 2496 / #ccffcc / signal sequence
                     Cleavage site after base 2427"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2497..2601)
                     /gene="bla"
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(2627..3082)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        3156..1
                     /note="CMV IE94 promoter"
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccactagt ggcgcgccat taattaaagg ccggccagcg
      121 atcgccggac atcgattgga attcaaggcc tctcgagcct ctagaactat agtgagtcgt
      181 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      241 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      301 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      361 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
      421 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      781 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      841 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      901 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      961 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1021 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1081 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1141 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1201 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1261 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1321 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1381 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1441 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1501 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1561 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     2641 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2701 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2761 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2821 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     2881 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     2941 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3001 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3061 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     3121 cgatcggtgc gggcctcttc gctattacgc cagtcgacca tagccaattc aatatggcgt
     3181 atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt
     3241 atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg
     3301 acttggcacc atgccaattc aatatggcgg acttggcact gtgccaactg gggaggggtc
     3361 tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt
     3421 gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat caatatatag
     3481 gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat actatgtatt
     3541 ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc
     3601 caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata gccactcccc
     3661 cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta
     3721 ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca
     3781 atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc
     3841 attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca
     3901 gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa ggacgccagg
     3961 gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa
     4021 gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg
     4081 taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaa