pMHE6廣宿主細(xì)菌表達(dá)體外表達(dá)載體-BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
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pMHE6 plasmid
BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心www.biovector.net
載體基本信息
| 出品人: | Fodor,B.D.(2004) |
|---|---|
| 載體名稱: | pMHE6 |
| 質(zhì)粒類型: | 廣宿主載體；細(xì)菌表達(dá)載體;體外表達(dá)載體 |
| 高拷貝/低拷貝: | -- |
| 克隆方法: | 限制性內(nèi)切酶，多克隆位點(diǎn) |
| 啟動(dòng)子: | T7/lac |
| 載體大小: | 5945 bp |
| 5' 測(cè)序引物及序列: | T7 promoter 5’TAATACGACTCACTATAGGG 3’ |
| 3' 測(cè)序引物及序列: | T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’ |
| 載體標(biāo)簽: | T7 tag（N-ter）；FLAG-tag、Strep-tag II (C-ter) |
| 載體抗性: | 鏈霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主細(xì)胞（系）: | 多個(gè)菌屬 |
| 備注: | -- |
| 產(chǎn)品目錄號(hào): | -- |
| 組成型/誘導(dǎo)型: | 誘導(dǎo)型 |
載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
載體簡(jiǎn)介
載體序列
LOCUS AY303672 5945 bp DNA circular SYN 10-FEB-2004
DEFINITION Broad host range expression vector pMHE6, complete sequence.
ACCESSION AY303672
VERSION AY303672.1 GI:34766426
KEYWORDS .
SOURCE Broad host range expression vector pMHE6
ORGANISM Broad host range expression vector pMHE6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5945)
AUTHORS Fodor,B.D., Kovacs,A.T., Csaki,R., Hunyadi-Gulyas,E., Klement,E.,
Maroti,G., Meszaros,L.S., Medzihradszky,K.F., Rakhely,G. and
Kovacs,K.L.
TITLE Modular broad-host-range expression vectors for single-protein and
protein complex purification
JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
PUBMED 14766546
REFERENCE 2 (bases 1 to 5945)
AUTHORS Fodor,B.D.
TITLE Direct Submission
JOURNAL Submitted (22-MAY-2003) Biophysics, BRC Hungarian Academy of
Sciences, Temesvari, Szeged, Csongrad 6701, Hungary
FEATURES Location/Qualifiers
source 1..5945
/organism="Broad host range expression vector pMHE6"
/mol_type="other DNA"
/db_xref="taxon:244947"
primer_bind 94..112
/note="T7 promoter primer"
regulatory 94..110
/regulatory_class="promoter"
/note="T7 promoter"
misc_feature 111..136
/note="lac operator"
misc_feature 182..214
/note="T7 tag"
misc_feature 278..301
/note="FLAG-tag"
misc_feature 287..301
/note="enterokinase cleaveage site"
misc_feature 305..328
/note="Strep-tag II"
primer_bind complement(382..399)
/note="T7 terminator primer"
regulatory 395..442
/regulatory_class="terminator"
/note="T7 terminator"
rep_origin 1074..1736
/note="pBBR1 oriV"
misc_feature 2730..3723
/note="RP4 mob, oriT"
regulatory complement(3813..3928)
/regulatory_class="terminator"
/note="T4 terminator"
gene complement(4188..4979)
/gene="aadA"
CDS complement(4188..4979)
/gene="aadA"
/note="streptomycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside-3:adenyltransferase fusion"
/protein_id="AAQ82545.1"
/db_xref="GI:34766427"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
regulatory 5715..5830
/regulatory_class="terminator"
/note="T4 terminator"
ORIGIN
1 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
61 gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt
121 gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca
181 tatggctagc atgactggtg gacagcaaat gggtcgggat ccgaattcga gctccgtcga
241 caagcttgat atcgaattcc tgcagctcga gggatccgac tacaaggacg acgacgacaa
301 gaactggagc catccgcagt tcgagaagtg aagatccggc tgctaacaaa gcccgaaagg
361 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
421 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg cccttcccaa
481 cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg
541 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgactgc gatgagtggc
601 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggtgctacgc
661 ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc
721 gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta
781 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag
841 gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg
901 ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
961 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc
1021 tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg
1081 cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
1141 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
1201 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg
1261 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc
1321 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac
1381 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac
1441 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg
1501 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg
1561 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc
1621 gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc
1681 cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt
1741 atctccatgc ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc tgcaactttt
1801 cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca gattttcttt
1861 aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta cccccctttt
1921 ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct ttggtgccca
1981 aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc tccggcaaaa
2041 agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc caaggtggcg
2101 ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg cgggcgggct
2161 tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag
2221 ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag
2281 cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg
2341 ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc
2401 ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca
2461 gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc
2521 cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca
2581 agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca aagtctagtg
2641 agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc cccgtcgagc
2701 cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg cgatgcaaga
2761 agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc gagcgcgaga
2821 cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc agcagcaccg
2881 atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag gacgctgtgt
2941 tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc agccaagaac
3001 agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac ggggcggatc
3061 gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc gcgttcgtgg
3121 tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac aaagcgcaga
3181 tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg caacggggca
3241 tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc ctggagcggc
3301 caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc tatgcaccgc
3361 agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa gccgtggccg
3421 accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc gccgcaggag
3481 cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgagac cttcgggagc
3541 gcctgaagcc cgttctggac gccctggggc cgttgaatcg ggatatgcag gccaaggccg
3601 ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa gtccagcgcc
3661 agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca tttccccgaa aagtgccacc
3721 tggcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga
3781 acgaattgtt aggtggcggt acttgggtcg atagctttat gcttgtaaac cgttttgtga
3841 aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc
3901 tattaaaggt cattcaaaag gtcatccacc ggatcagctt agtaaagccc tcgctagatt
3961 ttaatgcgga tgttgcgatt acttcgccaa ctattgcgat aacaagaaaa agccagcctt
4021 tcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc
4081 agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag
4141 ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattg ttagacatta tttgccgact
4201 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
4261 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
4321 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
4381 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
4441 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
4501 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
4561 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
4621 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
4681 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
4741 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
4801 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
4861 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
4921 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcatg
4981 atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
5041 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
5101 accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
5161 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagctta
5221 cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt
5281 cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag
5341 cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc
5401 aaggtgctgt gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg
5461 cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc
5521 gagcatcgtt tgttcgccca gcttctgtat ggaacgggca tgcggatcag tgagggtttg
5581 caactgcggg tcaaggatct ggatttcgat cacggcacga tcatcgtgcg ggagggcaag
5641 ggctccaagg atcgggcctt gatgttaccc gagagcttgg cacccagcct gcgcgagcag
5701 gggaattgat ccggtggatg accttttgaa tgacctttaa tagattatat tactaattaa
5761 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa cggtttacaa
5821 gcataaagct caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
5881 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
5941 tgtcg
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