pWPXLd慢病毒綠色熒光表達載體-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心
- 價 格：￥7920
- 貨 號：pWPXLd慢病毒綠色熒光表達載體
- 產 地：北京
- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
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pWPXLd慢病毒綠色熒光表達載體
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心www.biovector.net
EF1-alpha啟動子，Amp抗性。
pWPXLd is essentially pWPXL without the LoxP site in the 3' LTR.
pWPT or pWPXL can be used for constitutive transgene expression.
pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter
Unique restriction sites at key positions will allow you to change promoter and transgene. PmeI is a popular site for cloning. You can also use PacI and SwaI.
Please note that ClaI in these vectors is blocked by Dam methylation.
The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning.
Map圖譜
- Vector backbonepWPXLd(Search Vector Database)
Backbone size (bp)10455
- Vector typeMammalian Expression, Lentiviral
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
- Copy numberHigh Copy
GENE/INSERT
- Gene/Insert nameNone
Tag / Fusion Protein
EGFP
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteMCS - see map (not destroyed)
3′ cloning siteMCS - see map (not destroyed)
5′ sequencing primerSee map
BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心www.biovector.net
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