pHV1248， pHV1249 Tn10轉座子載體

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Map圖譜

Structures of pHV1248 and pHV1249. (A) Symbols: -, pBR322 sequence; E , pE194ts sequence; u, TnJO sequence; _,

pC194 sequence. ori denotes replication origin. Ampicillin (Ap), erythromycin (Em), and chloramphenicol (Cm) resistance genes are

indicated. The transposase (Tnase)-coding sequence is represented by an arrow, preceded by a black triangle for promoter and RBS fused

to it. Outside (0) and inside (I) ends of the transposon are indicated. Restriction sites for NcoI (L), EcoRI (A), and SstI (0) are depicted.

(B) Sequence upstream of the transposase gene. The tac promoter ptac, lac operator 0lac and RBS (derived from B. subtilis expression vector

p602-22; Le Grice, in press) are indicated.

Complete plasmid sequences are available upon request.