Walker256 cell line細胞株

BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心www.biovector.net

Organism: Rattus norvegicus, rat  /  Disease: mammary gland; Walker carcinomaCat No.: NTCC595169 Organism Rattus norvegicus, ratProduct Format frozenMorphology epithelialCulture Properties adherentBiosafety Level 1Disease mammary gland; Walker carcinomaStrain WalkerTumorigenic YesEffects Yes, in Amsterdam ratsVirus Resistance poliovirus 1Complete Growth Medium Medium 199, 95%; horse serum, 5% Subculturing Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommendedMedium Renewal: Twice per weekRemove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.References American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956.. . Science 117: 223, 1953.           BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心www.biovector.net      [Supplier來源]  http://www.biovector.net