Description: Cloning vector AbVec-hIgG1.

Keyword: .

Organism species: Cloning vector AbVec-hIgG1

Organism classification: other sequences; artificial sequences; vectors. organism: Cloning vector AbVec-hIgG1

note: expression cloning vector for human antibody

圖譜Map:

ID FJ475055; SV 1; circular; other DNA; STD; SYN; 5746 BP.序列Sequence:

AC   FJ475055;
DT   29-DEC-2008 (Rel. 98, Created)
DT   29-DEC-2008 (Rel. 98, Last updated, Version 1)
DE   Cloning vector AbVec-hIgG1, complete sequence.
KW   .
OS   Cloning vector AbVec-hIgG1
OC   other sequences; artificial sequences; vectors.
RN   [1]
RP   1-5746
RA   Wilson P.C.;
RT   "Antibody variable gene expression vector for human IgG1 heavy chain";
RL   Unpublished.
RN   [2]
RP   1-5746
RA   Wilson P.C.;
RT   ;
RL   Submitted (18-NOV-2008) to the EMBL/GenBank/DDBJ databases.
RL   Medicine/Rheumatology, The University of Chicago, BSLC/JFK Bldg. 924 East
RL   57th St., Rm 414, Chicago, IL 60637, USA
FH   Key             Location/Qualifiers
FT   source          1..5746
FT                   /organism="Cloning vector AbVec-hIgG1"
FT                   /mol_type="other DNA"
FT                   /note="expression cloning vector for human antibody
FT                   variable genes"
FT                   /db_xref="taxon:583342"
FT   promoter        1..618
FT                   /note="CMV"
FT   misc_feature    929..973
FT                   /note="murine IgG1 signal peptide; derived from
FT                   DEC205/immunoglobluin gamma fusion protein in GenBank
FT                   Accession Number DQ407610"
FT   misc_feature    974..991
FT                   /note="heavy chain variable gene cloning site; flanked by
FT                   AgeI and SalI sites"
FT   gene            <992..1984
FT                   /gene="IgG1"
FT   CDS             <992..1984
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="IgG1"
FT                   /product="IgG1"
FT                   /note="Cgamma-1; derived from Homo sapiens"
FT                   /protein_id="ACK87036.1"
FT                   /translation="RSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
FT                   LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD
FT                   KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD
FT                   GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA
FT                   KGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL
FT                   DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
FT   gene            3709..4569
FT                   /gene="Amp_Res"
FT   CDS             3709..4569
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="Amp_Res"
FT                   /product="beta-lactamase"
FT                   /note="confers resistance to ampicillin"
FT                   /protein_id="ACK87037.1"
FT                   /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
FT                   ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS
FT                   PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
FT                   EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
FT                   LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
FT                   LIKHW"
SQ   Sequence 5746 BP; 1400 A; 1564 C; 1399 G; 1383 T; 0 other;
ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca        60
ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct       120
ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta       180
acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac       240
ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt       300
aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag       360
tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat       420
gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat       480
gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc       540
ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt       600
ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga       660
caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt       720
gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta       780
gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac       840
tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc       900
acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt       960
agcaactgca accggtgtac actcgagcgt acggtcgacc aagggcccat cggtcttccc      1020
cctggcaccc tcctccaaga gcacctctgg gggcacagcg gccctgggct gcctggtcaa      1080
ggactacttc cccgaacctg tgacggtctc gtggaactca ggcgccctga ccagcggcgt      1140
gcacaccttc ccggctgtcc tacagtcctc aggactctac tccctcagca gcgtggtgac      1200
cgtgccctcc agcagcttgg gcacccagac ctacatctgc aacgtgaatc acaagcccag      1260
caacaccaag gtggacaaga aagttgagcc caaatcttgt gacaaaactc acacatgccc      1320
accgtgccca gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc      1380
caaggacacc ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag      1440
ccacgaagac cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc      1500
caagacaaag ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac      1560
cgtcctgcac caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc      1620
cctcccagcc cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca      1680
ggtgtacacc ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg      1740
cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc      1800
ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta      1860
cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt      1920
gatgcatgag gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa      1980
atgaagcttg gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa      2040
gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt      2100
tgtccaaact catcaatgta tcttatcatg tctggatcga tcgggaatta attcggcgca      2160
gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga      2220
aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca      2280
ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca      2340
ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc      2400
ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc      2460
catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta      2520
ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca      2580
gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac      2640
ttaatcgcct tgcagcacat ccccccttcg ccagctggcg taatagcgaa gaggcccgca      2700
ccgatcgccc ttcccaacag ttgcgtagcc tgaatggcga atggcgcctg atgcggtatt      2760
ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc      2820
gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac      2880
acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt      2940
cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc      3000
tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc      3060
gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact      3120
cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg      3180
gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc      3240
gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc      3300
tgatgccgca tagttaagcc aactccgcta tcgctacgtg actgggtcat ggctgcgccc      3360
cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct      3420
tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca      3480
ccgaaacgcg cgaggcagta ttcttgaaga cgaaagggcc tcgtgatacg cctattttta      3540
taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat      3600
gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg      3660
agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa      3720
catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac      3780
ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac      3840
atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt      3900
ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgatgacgcc      3960
gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca      4020
ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc      4080
ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag      4140
gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa      4200
ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc agcagcaatg      4260
gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa      4320
ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg      4380
gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt      4440
gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt      4500
caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag      4560
cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat      4620
ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct      4680
taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct      4740
tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca      4800
gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc      4860
agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc      4920
aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct      4980
gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag      5040
gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc      5100
tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg      5160
agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag      5220
cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt      5280
gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac      5340
gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg      5400
ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc      5460
cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata      5520
cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccagctggca cgacaggttt      5580
cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttacct cactcattag      5640
gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga      5700
taacaatttc acacaggaaa cagctatgac catgattacg aattaa                     5746

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