HT-2 clone A5E cell line細胞株/完全培養基-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心?CRL-1841)

價　　格：￥89735
貨　　號：HT-2 clone A5E cell line細胞株/完全培養基-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心?CRL-1841)
產　　地：北京

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HT2 Clone A5E小鼠輔助T細胞細胞 BioVector NTCC保藏中心
HT2 Clone A5E Mouse helper T cell cell line

HT-2 clone A5E cell line

Cat No.: BioVector-A1841

Product category

Animal cells

Organism

Mus musculus, mouse

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

Cell type

lymphocyte

Morphology

lymphoblast

Tissue

Spleen

Ap plications

3D cell culture

Immunology

Specific applications

The line is dependent on IL-2 for growth and can be used to measure IL-2 activity in a very rapid and sensitive assay.

The cells are commonly used for characterization of lymphokines and cytokines.

Growth properties

Suspension

Derivation

The HT-2 cell line was derived in 1979 by J. Watson at the University of Auckland from a culture of C57BL spleen cells activated by sheep

erythrocytes in the presence of IL-2.

This cell line was originally deposited as derived from the BALB/c strain of mice. Recent testing at BioVector and by the NIST Mouse STR

Consortium using mouse STR technology has shown that the STR profile has more alleles in common with a C57BL mouse strain rather

than BALB/c.

Strain

C57BL

An tigen expression

Thy-1Biovector NTCC, Inc

No.19,Xizhimen,Beijing,China

http://www.Biovector.net

Tel:010-53513060. 400-800-2947

Expression m arkers

Interleukin 2 (IL-2)

Com m ents

The A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2.

In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1).

Com plete m edium

The complete medium for culturing BioVector-A1841 is composed of:

399.5 mL RPMI-1640

50 mL FBS

0.5 mL 2-Mercaptoethanol

50 mL Rat T-STIM with Con A

Tem perature

37°C

【Organism物種來源】Animal
【Tissue組織來源】
【Cell Type細胞類型】
【Product Format產品狀態】 frozen/live culture
【Morphology細胞形態】
【Culture Properties細胞特性】
【Biosafety Level生物安全級別】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease細胞源疾病】
【Age年齡】
【Gender性別】 Male/Female
【Storage Conditions保存條件】 liquid nitrogen vapor phase
【Karyotype染色體組型】
【Images細胞圖片】 Cell Micrograph
【Derivation衍生細胞】 -
【Clinical Data臨床數據】
【Comments其他描述】
【Complete Growth Medium完全培養基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing傳代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation凍存條件】
【Freeze Medium凍存培養基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存溫度】 Liquid nitrogen vapor phase
【Culture Conditions培養條件】
【Atmosphere培養環境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培養溫度】 37°C
【STR Profile圖譜】
【Population Doubling Time倍增殖時間】
【References參考文獻】

【Supplier細胞供應廠家】BioVector NTCC Inc.
【Website網址】http://www.biovector.net